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Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals

Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher  excitation wavelength of red-shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for simultaneous real-time imaging of Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.

Here we provide transfection-grade Red-shifted Cameleons for the Cytosol, Mitochondria and the Endoplasmic Reticulum (ER):

D1CmR2 (cytosolic high KD)

D1CmR2 (cytosolic high KD)

 190.00

D1ERCmR2 (ER-targeted physiological KD)

D1ERCmR2 (ER-targeted physiological KD)

 190.00

D1ERCR (ER-targeted physiological KD)

D1ERCR (ER-targeted physiological KD)

 190.00

D1ERTG (ER-targete physiological KD)

D1ERTG (ER-targeted physiological KD)

 190.00

D3GO-Cam (cytosolic low KD)

D3GO-Cam (cytosolic low KD)

 190.00

mtD1CmR2 (mitochondrial high KD)

mtD1CmR2 (mitochondrial high KD)

 190.00

mtD1GO-Cam (mitochondrial physiological KD)

mtD1GO-Cam (mitochondrial physiological KD)

 190.00

mtD3GO-Cam (mitochondrial low KD)

mtD3GO-Cam (mitochondrial low KD)

 190.00

mtO-GECO1 (mitochondrial physiological KD)

mtO-GECO1 (mitochondrial physiological KD)

 190.00