MitoTools are a libary of FP-tagged components of the mitochondrial calcium uptake machinery. These fluorescent tools can be used to study the architecture and functioning of mitochondrial calcium uniport on the single cell level. E.g. FRET imaging between MICU1-CFP and MICU1-YFP was used to investigate the calcium dependent rearrangment of MICU1 oligomers. This is an essential step in the activation of MCU (see reference).
Here we provide the most important constituents of the MCU machinery as FP-tagged constructs in form of ready-to-use plasmid DNA