Nitric Oxide Probes

Novel Fluorescent Probes for live-cell imaging of Nitric Oxide

geNOps – genetically encoded nitric oxide (NO•) probes – are a novel class of fluorescent biosensors that allow real-time imaging of NO• dynamics on the single (sub)cellular level [Eroglu et al. Nat. Commun. 2016]. The NO• sensors are available in three different colors (cyan, green and orange) either localized in the cytosol or targeted to the mitochondrial matrix. As controls NO•-insensitive mutated constructs (ΔgeNOps) are offered. The novel NO• probes are available as optimized mammalian expression vectors for transfections and adeno viruses for delivering the geNOp-encoding DNA efficiently to biological samples of interest. For fast screening assays a cell line stably expressing green fluorescent geNOp (G-geNOp) is also offered.

Here we provide ready-to-use geNOps-encoding plasmids and related products in high quality for completing whole sets of experiments for live-cell imaging of Nitric Oxide:

 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00
 270.00

Chemical Tools

Booster solution for fast and non-toxic iron(II)-loading of cells

The Iron(II) booster solution has been optimized to load iron(II) into your cells of interest in a fast and non-toxic manner. Under standard cell culture conditions the iron(II) content of cells is suboptimal. Accordingly, processes that rely on this metal ion might be reduced in their activity. The geNOps technology demonstrated the power of the iron(II) booster solution for an efficient iron(II) loading.

Here we provide a ready-to-use buffer that increases the cellular iron(II) concentration within several minutes:

Iron(II) - booster solution

Iron(II) – booster solution

 70.00

Calcium Probes

Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals

Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher  excitation wavelength of red-shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for simultaneous real-time imaging of Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.

Here we provide transfection-grade Red-shifted Cameleons for the Cytosol, Mitochondria and the Endoplasmic Reticulum (ER):

D1CmR2 (cytosolic high KD)

D1CmR2 (cytosolic high KD)

 190.00

D1ERCmR2 (ER-targeted physiological KD)

D1ERCmR2 (ER-targeted physiological KD)

 190.00

D1ERCR (ER-targeted physiological KD)

D1ERCR (ER-targeted physiological KD)

 190.00

D1ERTG (ER-targete physiological KD)

D1ERTG (ER-targeted physiological KD)

 190.00

D3GO-Cam (cytosolic low KD)

D3GO-Cam (cytosolic low KD)

 190.00

mtD1CmR2 (mitochondrial high KD)

mtD1CmR2 (mitochondrial high KD)

 190.00

mtD1GO-Cam (mitochondrial physiological KD)

mtD1GO-Cam (mitochondrial physiological KD)

 190.00

mtD3GO-Cam (mitochondrial low KD)

mtD3GO-Cam (mitochondrial low KD)

 190.00

mtO-GECO1 (mitochondrial physiological KD)

mtO-GECO1 (mitochondrial physiological KD)

 190.00

ATP Probes for the ER

Genetically encoded FRET-based probes for imaging ATP within the Endoplasmic Reticulum (ER)

ERATs are genetically encoded FRET-based probes optimized for imaging ATP within the endoplasmic reticulum (ER) of mammlian cells [Vhisnu et al. Mol.Biol. Cell. 2013]. The ER ATP sensors are available either as CFP-YFP or GFP-RFP FRET-based probes. As controls mutated probes with either high or very low ATP sensitivity are also available.

Here we provide various ERAT-encoding plasmids for your research:

 210.00
 210.00

ERAT4.01

ERAT4.01

 210.00

ERAT4.01 N7Q

ERAT4.01 N7Q

 210.00

ERAT4.01 N7Q R122K R126K

ERAT4.01 N7Q R122K R126K (insensitive)

 210.00

ERAT4.01 N7Q YEMK

ERAT4.01 N7Q YEMK

 210.00

 

Mito Tools library

MitoTools are a library of FP-tagged components of the mitochondrial calcium uptake machinery. These fluorescent tools can be used to study the architecture and functioning of mitochondrial calcium uniport on the single cell level. E.g. FRET imaging between MICU1-CFP and MICU1-YFP was used to investigate the calcium dependent rearrangment of MICU1 oligomers. This is an essential step in the activation of MCU (see reference).

Here we provide the most important constituents of the MCU machinery as FP-tagged constructs in form of ready-to-use plasmid DNA

EMRE Constructs

hEMRE untagged

 150.00

hEMRE-Citrine

hEMRE-Citrine

 150.00
 150.00

EMRE Constructs

hEMRE-ECFP

 150.00

EMRE Constructs

hEMRE-mCherry

 150.00

hLETM1 untagged

hLETM1 untagged

 150.00

hLETM1-mCherry

hLETM1-mCherry

 150.00

hMCU -Citrine

hMCU -Citrine

 150.00

hMCUE257Q-mCherry

hMCU E257Q-mCherry

 150.00

hMCU untagged

hMCU untagged

 150.00

hMCU-ECFP

hMCU-ECFP

 150.00

hMCU-FRET

hMCU-FRET

 250.00

hMCUb -Citrine

hMCUb -Citrine

 150.00

hMCUb -ECFP

hMCUb -ECFP

 150.00

hMCUb -FRET

hMCUb -FRET

 250.00

hMCUb -mCherry

hMCUb -mCherry

 150.00

hMCUb untagged

hMCUb untagged

 150.00

hMCUR1-untagged

hMCUR1-untagged

 150.00

hMICU1-Citrine

hMICU1-Citrine

 150.00

hMICU1-ECFP

hMICU1-ECFP

 150.00

hMICU1-EFhand-mut-Citrine

hMICU1-EFhand-mut-Citrine

 150.00

hMICU1-EFhand-mut-ECFP

hMICU1-EFhand-mut-ECFP

 150.00

hMICU1-FRET

hMICU1-FRET

 250.00

hMICU1-untagged

hMICU1-untagged

 150.00

hUCP2-Citrine

hUCP2-Citrine

 150.00

hUCP2-FLAG

hUCP2-FLAG

 150.00

hUCP2-mCherry

hUCP2-mCherry

 150.00

hUCP2-R60K-FLAG

hUCP2-R60K-FLAG

 150.00

hUCP2-R71K-FLAG

hUCP2-R71K-FLAG

 150.00

hUCP2-untagged

hUCP2-untagged

 150.00

hUCP3-Citrine

hUCP3-Citrine

 150.00

hUCP3-ECFP

hUCP3-ECFP

 150.00

hUCP3-FRET

hUCP3-FRET

 250.00

hUCP3-R167G

hUCP3-R167G

 150.00

hUCP3-R171G-E172G

hUCP3-R171G-E172G

 150.00

hUCP3-untagged

hUCP3-untagged

 150.00

rLetm1-mCherry

rLetm1-mCherry

 150.00

rMcu-mCherry

rMcu-mCherry

 150.00

rMicu1-mCherry

rMicu1-mCherry

 150.00

rUcp2-mCherry

rUcp2-mCherry

 150.00

sECFP-hMCU

sECFP-hMCU

 150.00

sECFP-hMICU1

sECFP-hMICU1

 150.00