Novel Genetically Encoded Potassium Probes for live-cell imaging
GEPIIs– genetically encoded potassium ion indicators – are a novel class of genetically encoded fluorescent biosensors that enable real-time imaging of Potassium ion fluctuations on the single (sub)cellular level [Bischof et al. Nat. Commun. 2017]. Differentially targeted GEPIIs are either localized in the cytosol or targeted to the mitochondrial matrix or in the subplasma region. We also offer GEPIIs with different sensitivities for high resolution detection of Potassium. The novel Potassium probes are available as optimized mammalian expression vectors driven by an CMV promotor.
Here we provide ready-to-use GEPII-encoding plasmids in high quality for live-cell imaging of Potassium fluctuations:
Nitric Oxide Probes
Novel Fluorescent Probes for live-cell imaging of Nitric Oxide
geNOps – genetically encoded nitric oxide (NO•) probes – are a novel class of fluorescent biosensors that allow real-time imaging of NO• dynamics on the single (sub)cellular level [Eroglu et al. Nat. Commun. 2016]. The NO• sensors are available in three different colors (cyan, green and orange) either localized in the cytosol or targeted to the mitochondrial matrix. As controls NO•-insensitive mutated constructs (ΔgeNOps) are offered. The novel NO• probes are available as optimized mammalian expression vectors for transfections and adeno viruses for delivering the geNOp-encoding DNA efficiently to biological samples of interest. For fast screening assays a cell line stably expressing green fluorescent geNOp (G-geNOp) is also offered.
Here we provide ready-to-use geNOps-encoding plasmids and related products in high quality for completing whole sets of experiments for live-cell imaging of Nitric Oxide:
Booster solution for fast and non-toxic iron(II)-loading of cells
The Iron(II) booster solution has been optimized to load iron(II) into your cells of interest in a fast and non-toxic manner. Under standard cell culture conditions the iron(II) content of cells is suboptimal. Accordingly, processes that rely on this metal ion might be reduced in their activity. The geNOps technology demonstrated the power of the iron(II) booster solution for an efficient iron(II) loading.
Here we provide a ready-to-use buffer that increases the cellular iron(II) concentration within several minutes:
Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals
Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher excitation wavelength of red-shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for simultaneous real-time imaging of Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.
Here we provide transfection-grade Red-shifted Cameleons for the Cytosol, Mitochondria and the Endoplasmic Reticulum (ER):
ATP Probes for the ER
ATP Probes for the ER
Genetically encoded FRET-based probes for imaging ATP within the Endoplasmic Reticulum (ER)
ERATs are genetically encoded FRET-based probes optimized for imaging ATP within the endoplasmic reticulum (ER) of mammlian cells [Vhisnu et al. Mol.Biol. Cell. 2013]. The ER ATP sensors are available either as CFP-YFP or GFP-RFP FRET-based probes. As controls mutated probes with either high or very low ATP sensitivity are also available.
Here we provide various ERAT-encoding plasmids for your research:
Mito Tools library
MitoTools are a library of FP-tagged components of the mitochondrial calcium uptake machinery. These fluorescent tools can be used to study the architecture and functioning of mitochondrial calcium uniport on the single cell level. E.g. FRET imaging between MICU1-CFP and MICU1-YFP was used to investigate the calcium dependent rearrangment of MICU1 oligomers. This is an essential step in the activation of MCU (see reference).
Here we provide the most important constituents of the MCU machinery as FP-tagged constructs in form of ready-to-use plasmid DNA