The AnglerFish is a multi-use 3LED and 2CAMs inverted widefield fluorescence-microscope.
With its different configuration options, the variety of its use ranges from 2LED-1CAM-setup for e.g. FURA-2-measurements or 1LED-2CAMs-setup for e.g. CFP-YFP FRET-measurements to 3LED-2CAMs-setup for e.g. FURA-2 /GFP-OFP FRET-measurements.
The different measuring configurations are provided with their corresponding sets of LEDs, dichroic mirrors and emission filters which are put in their corresponding positions according to the provided assembly diagram.
The software needed to run the AnglerFish is provided via USB-installer and needs a 64 bit Windows10 System. The AnglerFish does not require much installation. Just install the software, connect it with the computer via a USB-cable and place the power supply.
With the AnglerFish software all AnglerFish functions are controllable like:
- LED intensity
- Motorized Positioning of the xyz-stage
- Camera Control like Exposure, Binning, Gain
- Setting of ROIs and creation of ROI-graphs and Ratio-graphs
- Time lapse programming
up to 4 Live-time intensity and 2 Ratio-graphs simultaneously
With the AnglerFish software you can create different imaging settings for your measurements depending on your AnglerFish hardware setup and run them in time lapse mode with live camera view and ROI- and Ratio-graphs while the obtained pictures are saved for picture processing.
Pictures will be saved as TIFF and ROI data will be exported in Excel files.
For more information and pricing please contact us.
AnglerFish mtGCaMP plus Fura2- measurement
AnglerFish Lab at Medical University of Graz