ERAT4.01 N7Q R122K R126K (insensitive)

ERAT4.01 N7Q R122K R126K is a CFP-YFP-FRET-based probe that is ATP insensitive with a targeting seqeunce for the ER. It can be used as a negative control in combination with either ERAT4.01 or ERAT4.01 N7Q (lacking a putative glycosylation site).

ERAT4.01 N7Q R122K R126K use
In order to express ERAT4.01 N7Q R122K R126K in cells of interest 20 µg purified, endotoxin-free plasmid DNA coding for the ATP insensitive construct is provided. The plasmid coding for ERAT4.01 N7Q R122K R126K represents a mammalian expression vector with a strong viral promotor. For plasmid amplification in E.coli. ampicillin should be used. Vector maps will be provided upon request. 1 – 1.5 µg DNA is required for cell transfection in a single well of a standard 6-well dish following standard transfection procedures. Usually cells express high amounts of ERAT4.01 N7Q R122K R126K within 24 – 48 hours after cell transfection. Standard optical filters for CFP-YFP FRET imaging should be used.