Potassium Probes

Novel Genetically Encoded Potassium Probes for live-cell imaging 

GEPIIs– genetically encoded potassium ion indicators – are a novel class of genetically encoded fluorescent biosensors that enable real-time imaging of Potassium ion fluctuations on the single (sub)cellular level [Bischof et al. Nat. Commun. 2017]. Differentially targeted GEPIIs are either localized in the cytosol or targeted to the mitochondrial matrix or in the subplasma region. We also offer GEPIIs with different sensitivities for high resolution detection of Potassium. The novel Potassium probes are available as optimized mammalian expression vectors driven by an CMV promotor.

Here we provide ready-to-use GEPII-encoding plasmids in high quality for live-cell imaging of Potassium fluctuations:

GEPII (Potassium Probes)

GEPII 1.0 (cytosolic)

 270.00 excl.VAT
 270.00 excl.VAT

GEPII (Potassium Probes)

lc-LysM GEPII 1.0 (cytosolic)

 270.00 excl.VAT

Nitric Oxide Probes

Novel Fluorescent Probes for live-cell imaging of Nitric Oxide

geNOps – genetically encoded nitric oxide (NO•) probes – are a novel class of fluorescent biosensors that allow real-time imaging of NO• dynamics on the single (sub)cellular level [Eroglu et al. Nat. Commun. 2016]. The NO• sensors are available in three different colors (cyan, green and orange) either localized in the cytosol or targeted to the mitochondrial matrix. As controls NO•-insensitive mutated constructs (ΔgeNOps) are offered. The novel NO• probes are available as optimized mammalian expression vectors for transfections and adeno viruses for delivering the geNOp-encoding DNA efficiently to biological samples of interest. For fast screening assays a cell line stably expressing green fluorescent geNOp (G-geNOp) is also offered.

Here we provide ready-to-use geNOps-encoding plasmids and related products in high quality for completing whole sets of experiments for live-cell imaging of Nitric Oxide:

 270.00 excl.VAT
 270.00 excl.VAT
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 270.00 excl.VAT
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 270.00 excl.VAT
 270.00 excl.VAT
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 270.00 excl.VAT
 270.00 excl.VAT

Novel Fluorescent Probes for Ratiometric (Sub)cellular pH Imaging

pH-Lemons – genetically encoded FRET-based pH probes for (sub)cellular (cytosol, autophagosomes and lysosomes, the secretory pathway and cell surface) imaging of pH dynamics of neutral to acidic variations [Burgstaller Sandra et al. ACS Sensors 2019]. Here we provide ready-to-use pH-Lemon-encoding plasmids and related products in high quality for pH imaging experiments:

pH Lemon - cytosolic

pH Lemon cytosolic

 270.00 excl.VAT

pH Lemon - GPI

pH Lemon GPI

 270.00 excl.VAT

pH Lemon - LC3B

pH Lemon LC3B

 270.00 excl.VAT

Chemical Tools

Booster solution for fast and non-toxic iron(II)-loading of cells

The Iron(II) booster solution has been optimized to load iron(II) into your cells of interest in a fast and non-toxic manner. Under standard cell culture conditions the iron(II) content of cells is suboptimal. Accordingly, processes that rely on this metal ion might be reduced in their activity. The geNOps technology demonstrated the power of the iron(II) booster solution for an efficient iron(II) loading.

Here we provide a ready-to-use buffer that increases the cellular iron(II) concentration within several minutes:

Iron(II) - booster solution

Iron(II) – booster solution

 70.00 excl.VAT

Calcium Probes

Red-shifted Cameleons for live-cell imaging of intracellular Ca2+ signals

Red-shifted Cameleons are FRET-based Ca2+ biosensors that consist of a green donor and an orange or red acceptor fluorescent protein, respectively. The probes are optimized for measuring Ca2+ in the cytosol, in the mitochondrial matrix or in the lumen of the endoplasmic reticulum. The higher  excitation wavelength of red-shifted cameleons (~ 480 nm) allows a combination with UV-excitable chemical indicators such as fura-2 for simultaneous real-time imaging of Ca2+ dynamic within organelles and the cytosol in a ratiometric manner.

Here we provide transfection-grade Red-shifted Cameleons for the Cytosol, Mitochondria and the Endoplasmic Reticulum (ER):

D1CmR2 (cytosolic high KD)

D1CmR2 (cytosolic high KD)

 190.00 excl.VAT

D1ERCmR2 (ER-targeted physiological KD)

D1ERCmR2 (ER-targeted physiological KD)

 190.00 excl.VAT

D1ERCR (ER-targeted physiological KD)

D1ERCR (ER-targeted physiological KD)

 190.00 excl.VAT

D1ERTG (ER-targete physiological KD)

D1ERTG (ER-targeted physiological KD)

 190.00 excl.VAT
 190.00 excl.VAT

D3GO-Cam (cytosolic low KD)

D3GO-Cam (cytosolic low KD)

 190.00 excl.VAT

mtD1CmR2 (mitochondrial high KD)

mtD1CmR2 (mitochondrial high KD)

 190.00 excl.VAT

mtD1GO-Cam (mitochondrial physiological KD)

mtD1GO-Cam (mitochondrial physiological KD)

 190.00 excl.VAT

mtD3GO-Cam (mitochondrial low KD)

mtD3GO-Cam (mitochondrial low KD)

 190.00 excl.VAT

mtO-GECO1 (mitochondrial physiological KD)

mtO-GECO1 (mitochondrial physiological KD)

 190.00 excl.VAT

ATP Probes for the ER

Genetically encoded FRET-based probes for imaging ATP within the Endoplasmic Reticulum (ER)

ERATs are genetically encoded FRET-based probes optimized for imaging ATP within the endoplasmic reticulum (ER) of mammlian cells [Vhisnu et al. Mol.Biol. Cell. 2013]. The ER ATP sensors are available either as CFP-YFP or GFP-RFP FRET-based probes. As controls mutated probes with either high or very low ATP sensitivity are also available.

Here we provide various ERAT-encoding plasmids for your research:

 210.00 excl.VAT
 210.00 excl.VAT
 210.00 excl.VAT
 210.00 excl.VAT
 210.00 excl.VAT

ERAT4.01

ERAT4.01

 210.00 excl.VAT

ERAT4.01 N7Q

ERAT4.01 N7Q

 210.00 excl.VAT

ERAT4.01 N7Q R122K R126K

ERAT4.01 N7Q R122K R126K (insensitive)

 210.00 excl.VAT

ERAT4.01 N7Q YEMK

ERAT4.01 N7Q YEMK

 210.00 excl.VAT

 

Mito Tools library 

MitoTools are a library of FP-tagged components of the mitochondrial calcium uptake machinery. These fluorescent tools can be used to study the architecture and functioning of mitochondrial calcium uniport on the single cell level. E.g. FRET imaging between MICU1-CFP and MICU1-YFP was used to investigate the calcium dependent rearrangment of MICU1 oligomers. This is an essential step in the activation of MCU (see reference).

Here we provide the most important constituents of the MCU machinery as FP-tagged constructs in form of ready-to-use plasmid DNA

EMRE Constructs

hEMRE untagged

 150.00 excl.VAT

hEMRE-Citrine

hEMRE-Citrine

 150.00 excl.VAT

Uncategorized

hEMRE-ECFP

 150.00 excl.VAT

EMRE Constructs

hEMRE-ECFP

 150.00 excl.VAT

EMRE Constructs

hEMRE-mCherry

 150.00 excl.VAT

hLETM1 untagged

hLETM1 untagged

 150.00 excl.VAT

hLETM1-mCherry

hLETM1-mCherry

 150.00 excl.VAT

hMCU -Citrine

hMCU -Citrine

 150.00 excl.VAT

hMCUE257Q-mCherry

hMCU E257Q-mCherry

 150.00 excl.VAT

hMCU untagged

hMCU untagged

 150.00 excl.VAT

hMCU-ECFP

hMCU-ECFP

 150.00 excl.VAT

hMCU-FRET

hMCU-FRET

 250.00 excl.VAT